Figure 2

Identification of migrated cells, and morphological changes of transplanted adipose tissue-derived stromal cells (ASCs) and bone marrow-derived stromal cells (ST2). (A) Immunostaining of Gr-1 (red) at 1, 3, and 7 days after perforation and green fluorescent protein-positive (GFP⁺) bone marrow cell (BMC) migration (green). Scale bars = 50 μm. (B) Immunostaining of F4/80 (red) at 1, 3, and 7 days after perforation and GFP⁺ BMC migration (green). Scale bars = 50 μm. (C) Immunostaining of Gr-1 (red) at 1 day after transplantation of ASCs or ST2 cells and GFP⁺ BMC migration (green). Scale bars = 100 μm. (D) Immunostaining of F4/80 (red) at 7 days after transplantation of ASCs or ST2 cells and migration of GFP⁺ BMCs (green). Scale bars = 100 μm. (E) Immunostaining of CD11c (red) at 3 days after transplantation of ASCs or ST2 cells and GFP⁺ BMC migration (green). Scale bars = 100 μm. (F) Morphological changes of PKH26-labeled ASCs and ST2 cells (red) on days 3 and 7 after transplantation. Transplanted cells were engrafted after 3 days but were disrupted after 7 days. Arrows show lipids from PKH26 (lipophilic dye) linked membranes of burst cells. Scale bars = 50 μm. Nuclear staining was performed using Hoechst 33258 (blue).