Figure 1From: Susceptibility of murine induced pluripotent stem cell-derived cardiomyocytes to hypoxia and nutrient deprivationDifferentiation of murine induced pluripotent stem cells (iPSCs) into cardiomyocytes (CMs). (A) Schematic representation of the experimental protocol. (B) Representative images of embryoid bodies (EBs) composed of a mixed cell population containing areas of GFP-expressing CMs on differentiation day 9. Purified cardiac clusters were generated by selection with puromycin until day 16. (C) Flow cytometry analysis of GFP-positive iPS-CMs after trypsinization of puromycin-selected cardiac clusters on day 16. (D) α-Actinin (red) staining of puromycin-selected CMs (green) on differentiation day 21. (E) Effect of hypoxia and glucose/serum deprivation on poly-caspase activity in iPS-CMs. The baseline level of poly-caspase active in iPS-CMs was determined in normoxia and full medium. *P ≤ 0.05, versus normoxia for individual datasets (Bonferroni test); **P < 0.001, overall by analysis of variance. FBS, fetal bovine serum; GFP, green fluorescent protein; Glu, glucose; PI, propidium iodide.Back to article page