Reactive oxygen species (ROS) expression analysis. (A) Representative images of ROS accumulation in cells with exacerbated oxidative stress, visualized using the CellROX assay®. In induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) and neonatal cardiomyocytes (N-CMs) after 3 hours hypoxia and glucose/serum deprivation (GSD) compared to normoxia control (21% O2, 15% fetal bovine serum and high glucose). Scale bar 100 μm. (B) Quantification of oxidative stress (CellROX® fluorescence intensity) in iPS-CMs and N-CMs cultured in normoxia versus those cultured in hypoxia and GSD (#
P = 0.005, versus normoxia control; ##
P = 0.002, iPS-CMs versus N-CMs in hypoxia).