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Figure 12 | Stem Cell Research & Therapy

Figure 12

From: Susceptibility of murine induced pluripotent stem cell-derived cardiomyocytes to hypoxia and nutrient deprivation

Figure 12

Reactive oxygen species (ROS) expression analysis. (A) Representative images of ROS accumulation in cells with exacerbated oxidative stress, visualized using the CellROX assay®. In induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) and neonatal cardiomyocytes (N-CMs) after 3 hours hypoxia and glucose/serum deprivation (GSD) compared to normoxia control (21% O2, 15% fetal bovine serum and high glucose). Scale bar 100 μm. (B) Quantification of oxidative stress (CellROX® fluorescence intensity) in iPS-CMs and N-CMs cultured in normoxia versus those cultured in hypoxia and GSD (# P = 0.005, versus normoxia control; ## P = 0.002, iPS-CMs versus N-CMs in hypoxia).

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