Analysis of mitochondrial respiration of induced pluripotent stem cell-derived cardiomyocytes using the Seahorse XF Cell Mito Stress Kit. (A) Oxygen consumption rate (OCR) under basal and under different conditions of mitochondrial stress that was induced by addition of substances indicated on top of the panel. Three cycles of measurements were performed for each condition. (B) Individual mitochondrial function parameters calculated from the data shown in (A). Data in (A) and (B) are shown as mean ± SEM for n = 7 replicates. FCCP, carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone; non-Mito, non-mitochondrial oxygen consumption.