|
Day 1
|
M0
|
M1
|
M2
|
|---|
| |
Mean
|
P
Value
|
Mean
|
P
Value
|
Mean
|
P
Value
|
|
TNF
|
20 ± 7.8
|
0.000
|
102 ± 86.3
|
0.001
|
49 ± 31.3
|
0.000
|
|
IL-1β
|
21 ± 12.3
|
0.001
|
2791 ± 1765.2
|
0.000
|
86 ± 117.9
|
0.002
|
|
COX2
|
1.3 ± 0.8
|
0.942
|
4.9 ± 4.0
|
0.032
|
1.1 ± 0.6
|
0.915
|
|
MMP1a
|
3.0 ± 1.3
|
0.008
|
150 ± 90.5
|
0.000
|
5.5 ± 5.3
|
0.011
|
|
MMP3
|
6.3 ± 6.0
|
0.179
|
111 ± 58.8
|
0.000
|
5.1 ± 3.8
|
0.054
|
|
MMP13
|
1.8 ± 1.1
|
0.044
|
46 ± 12.0
|
0.000
|
2.0 ± 0.8
|
0.027
|
|
DCN
|
−1.3 ± 0.1
|
0.019
|
4.3 ± 2.7
|
0.006
|
−1.0 ± 0.3
|
0.689
|
|
BGN
|
−1.4 ± 0.1
|
0.002
|
−1.7 ± 0.1
|
0.001
|
−1.4 ± 0.1
|
0.004
|
|
COL1
|
−1.7 ± 0.1
|
0.001
|
−3.4 ± 0.0
|
0.000
|
−1.9 ± 0.1
|
0.001
|
|
COL3
|
−1.3 ± 0.2
|
0.082
|
−2.8 ± 0.1
|
0.000
|
−1.6 ± 0.1
|
0.002
|
|
SCX
|
1.2 ± 0.5
|
0.660
|
−1.3 ± 0.3
|
0.122
|
−1.2 ± 0.3
|
0.201
|
|
TNMD
|
1.0 ± 0.5
|
0.796
|
−2.9 ± 0.2
|
0.003
|
−1.4 ± 0.3
|
0.124
|
- mRNA expression of TFs after one day of co-culture with macrophages (M0, M1, M2) are represented as fold changes compared to untreated TFs (mean ± SD). Of the three macrophage phenotypes, M1 macrophages had a significantly greater effect on TF gene expression than M0 or M2. There was a significant effect of macrophage type for all factors (indicated by bold font; N = 5). BGN, biglycan; COL, collagen; COX, cyclooxygenase; DCN, decorin; IL-1β, interleukin-1β; MMP, matrix metalloproteinase; SCX, scleraxis; TFs, tendon fibroblasts; TNF, tumor necrosis factor; TNMD, tenomodulin.
