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Table 1 Macrophages induced up-regulation of pro-inflammatory and matrix degradation factors and down-regulation of matrix production- and tendon differentiation-related factors by TFs

From: Adipose-derived mesenchymal stromal cells modulate tendon fibroblast responses to macrophage-induced inflammation in vitro

Day 1 M0 M1 M2
  Mean P Value Mean P Value Mean P Value
TNF 20 ± 7.8 0.000 102 ± 86.3 0.001 49 ± 31.3 0.000
IL-1β 21 ± 12.3 0.001 2791 ± 1765.2 0.000 86 ± 117.9 0.002
COX2 1.3 ± 0.8 0.942 4.9 ± 4.0 0.032 1.1 ± 0.6 0.915
MMP1a 3.0 ± 1.3 0.008 150 ± 90.5 0.000 5.5 ± 5.3 0.011
MMP3 6.3 ± 6.0 0.179 111 ± 58.8 0.000 5.1 ± 3.8 0.054
MMP13 1.8 ± 1.1 0.044 46 ± 12.0 0.000 2.0 ± 0.8 0.027
DCN −1.3 ± 0.1 0.019 4.3 ± 2.7 0.006 −1.0 ± 0.3 0.689
BGN −1.4 ± 0.1 0.002 −1.7 ± 0.1 0.001 −1.4 ± 0.1 0.004
COL1 −1.7 ± 0.1 0.001 −3.4 ± 0.0 0.000 −1.9 ± 0.1 0.001
COL3 −1.3 ± 0.2 0.082 −2.8 ± 0.1 0.000 −1.6 ± 0.1 0.002
SCX 1.2 ± 0.5 0.660 −1.3 ± 0.3 0.122 −1.2 ± 0.3 0.201
TNMD 1.0 ± 0.5 0.796 −2.9 ± 0.2 0.003 −1.4 ± 0.3 0.124
  1. mRNA expression of TFs after one day of co-culture with macrophages (M0, M1, M2) are represented as fold changes compared to untreated TFs (mean ± SD). Of the three macrophage phenotypes, M1 macrophages had a significantly greater effect on TF gene expression than M0 or M2. There was a significant effect of macrophage type for all factors (indicated by bold font; N = 5). BGN, biglycan; COL, collagen; COX, cyclooxygenase; DCN, decorin; IL-1β, interleukin-1β; MMP, matrix metalloproteinase; SCX, scleraxis; TFs, tendon fibroblasts; TNF, tumor necrosis factor; TNMD, tenomodulin.