Influence of vascular endothelial growth factor stimulation and serum deprivation on gene activation patterns of human adipose tissue-derived stromal cells

Table 3 Expression levels for regulated genes according to primary gene ontology function

		Serum-deprived			VEGF-stimulated
Gene ID	Gene name	FC	2 ^\|FC\|		FC	2 ^\|FC\|
*Proliferation*
SPRY1	Sprouty homolog 1, antagonist of FGF signaling	+1.493		+2.815	NS		NS
TEK	TEK tyrosine kinase, endothelial	+1.024		+2.033	NS		NS
JAG1	Jagged 1	+0.8085		+1.751	+0.9621		+1.948
CDKN3	Cyclin-dependent kinase inhibitor 3	−1.668		−3.178	−1.386		−2.614
MKI67	Antigen identified by monoclonal antibody Ki-67	NS		NS	−1.222		−2.333
CCNB1	Cyclin B1	−1.324		−2.504	NS		NS
HMOX1	Heme oxygenase (decycling) 1	−1.047		−2.066	−0.969		−1.957
TPX2	TPX2, microtubule-associated	NS		NS	−1.042		−2.058
*Secretome*
IGF1	Insulin-like growth factor 1	+1.963		+3.897	+1.999		+3.998
BMP6	Bone morphogenetic protein 6	+1.902		+3.738	+1.980		+3.944
TRIL	TLR4 interactor with leucine-rich repeats	+1.691		+3.229	+2.005		+4.013
PCSK5	Proprotein convertase subtilisin/kexin type 5	+1.091		+2.131	+1.480		+2.791
FGF9	Fibroblast growth factor 9	+1.044		+2.061	+0.871		+1.829
HGF	Hepatocyte growth factor	NS		NS	+0,9719		+1,961
PDGFD	Platelet derived growth factor D	+1.048		+2.067	+0.900		+1.866
GREM1	Gremlin 1, DAN family BMP antagonist	−0.960		−1.946	−0.918		−1.891
TGF1	Transforming growth factor beta1	−0.962		−1.965	−0.908		−1.877
MME	Membrane metallo-endopeptidase	−1.322		−2.500	−1.432		−2.699
GREM2	Gremlin 2, DAN family BMP antagonist	−2.326		−5.019	−2.425		−5.368

Pre-defined groups. Fold changes (FC) and consequently calculated amount of up (+) or down (−) regulation (2^|FC|) of significant genes (P < 0.05) compared to adipose tissue-derived stromal cells (ASCs) in complete medium are listed for vascular endothelial growth factor (VEGF)-treated and serum-deprived ASCs FC >0. 9. NS, not significant.

ISSN: 1757-6512