Figure 13

Effects of implantation of DFAT cells with TSG-6 siRNA on degeneration of renal cortex in mAb 1-22-3-induced glomerulonephritis. Wistar rats were injected with 0.5 mg of mAb 1-22-3 and saline or 10⁶ DFAT cells transfected with 20 nM TSG-6 siRNA or 20 nM control siRNA through the tail vein. Fourteen days after the injections, all rats were killed and the left kidney was removed. A) The paraffin sections of removed renal cortex were stained with hematoxylin and eosin (H & E). Renal cortical thickness was measured under high magnification (x400). B) Glomerular injury score (GIS). C) Tubulointerstitial injury score (TIS). Data are the mean ± SEM (n = 4). **P <0.01 in the indicated columns. Bar = 50 μm. DFAT, dedifferentiated fat; mAb, monoclonal antibody; SEM, standard error of the mean; TSG-6, TNF-stimulated gene-6.