Stemness-related properties of granulation tissue-derived cells. Passage 2 granulation tissue-derived cells (GTCs) or nonwounded dermal cells were used for experiments. (A) The proliferation ability of GTCs and nonwounded dermal cells was detected every 2 days after seeding. (B) Colony-forming assay of GTCs and nonwounded dermal cells. Results expressed as mean ± standard deviation (n = 6 wells per group). (C) Adipogenic differentiation was induced in mouse mesenchymal stem cell adipogenic differentiation medium. After 22 days, cells were fixed and stained by Oil-Red O. The Oil-Red O staining was also quantified. (D) Osteogenic differentiation was performed in mouse mesenchymal stem cell osteogenic differentiation medium. After 21 days, cells were fixed and stained by Alizarin Red. The Alizarin Red staining was also quantified. d, days; OD, optical density. **P <0.01. Scale bar = 500 μm.