Figure 1From: Mouse aorta-derived mesenchymal progenitor cells contribute to and enhance the immune response of macrophage cells under inflammatory conditionsmAo cells in culture with MΦ cells contribute to local NO production in response to LPS and LPS/IFNγ. Nitrite production as a measure of NO in culture supernatants of mAo, MΦ, and splenic MΦ cells derived from TLR4-deficient mice (TLR4-MΦ) treated (A) with LPS (100 ng/mL) and (B) with LPS (100 ng/mL) + IFNγ (250 ng/mL) alone and in co-culture. Data are presented as mean ± standard error of the mean and are representative of three experiments each with n = 4. *Significantly different from MΦ cells alone; #significantly different from TLR4-MΦ cells alone. (C) Representative Western blots from a separate experiment showing iNOS expression. Whole lysates were prepared from cultures, and 50 μg was loaded per lane. β-actin was used to control for protein loading. IFNγ, interferon-gamma; iNOS, inducible nitric oxide synthase; LPS, lipopolysaccharide; MΦ, bone marrow-derived macrophage; mAo, mouse aorta-derived mesenchymal progenitor; MSC, mesenchymal stem cell; NO, nitric oxide; TLR4, Toll-like receptor-4; TLR4-MΦ, Toll-like receptor-4-deficient macrophage.Back to article page