Figure 5

MΦ and mAo cell interaction restores ox-LDL-suppressed local LPS- and LPS/IFNγ-induced cytokine/chemokine production. Mouse cytokine Proteome Profiler immunoblots (A) and corresponding graphs of densitometry results (B, C) demonstrate changes in cytokine/chemokine levels in supernatants of MΦ cells, MΦ cells exposed to ox-LDL (50 μg/mL), and MΦ cells exposed to ox-LDL in co-culture with mAo cells. Cultures were activated with LPS (100 ng/mL) and LPS (100 ng/mL) + IFNγ (250 ng/mL) for 24 hours. Numbered boxes highlight cytokine/chemokines whose expression is changed with treatments in (A) and correspond with cytokine/chemokine numbers in (B) and (C). Circles highlight internal control spots used to normalize densitometric data. Graphs in (B) and (C) present the changes in spot density when compared with untreated MΦ cells (Fold Δ/UNT MΦ). The blot of the untreated MΦ cells can be viewed in Additional file 2: Figure S2 along with the overlay. The key for the spots can be found in Additional file 3. CXCL, chemokine (C-X-C motif) ligand; G-CSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte/macrophage colony-stimulating factor; IFNγ, interferon-gamma; IL, interleukin; LPS, lipopolysaccharide; MΦ, bone marrow-derived macrophage; mAo, mouse aorta-derived mesenchymal progenitor; MCP, monocyte chemoattractant protein; ox-LDL, oxidized low-density lipoprotein; RANTES, regulated on activation, normal T-cell expressed and secreted; sICAM1, soluble intercellular adhesion molecule-1; TNFα, tumor necrosis factor-alpha.