Figure 7

Macrophage migration inhibitory factor restores cell survival through CD74. (A) Representative distributions of propidium iodide (PI) and Annexin V staining from FACScan flow cytometric analyses of apoptotic cells in normal and hypoxia and serum deprivation (hypoxia/SD) (6 hours) conditions, in cultures of untransfected and untreated mesenchymal stem cells (MSCs), and macrophage migration inhibitory factor (MIF)-treated (100 ng/ml at the point of exposure to hypoxia/SD) control MSCs, CD74-small interfering RNA (siRNA) transfected MSCs and scrambled small interfering RNA (siRNA-NT) transfected MSCs. MIF was added to the incubation medium throughout the hypoxia/SD treatment period. (B) Fold-change of apoptotic cells in above conditions, compared with control. Each column represents mean ± standard deviation from three independent experiments; *P <0.05 versus control; ^△ P <0.05 versus hypoxia/SD; ^◇ P <0.05 versus hypoxia/SD + siRNA-CD74.