Figure 1
From: Local injection of mesenchymal stem cells protects testicular torsion-induced germ cell injury
Orbital fat-derived stem cells rescued torsion–detorsion-induced spermatogenic failure. (A) Rats received surgical 720° testicular torsion for 3 hours on the left testis, and 3 × 104 human orbital fat stem cells (OFSCs) in 20 μl phosphate-buffered saline was administrated via local injection to the left testis 30 minutes before detorsion. Blood samples were collected on days 0, 3 and 7. Animals were sacrificed for orchiectomy of both testes on day 7. Tissue section of left testis showed (B) a normal spermatogenesis in the sham operation group (Ctrl), (C) a poor spermatogenesis after torsion–detorsion (T/D), and (D) mature sperm formation after T/D with OFSC injection. (E) Johnsen’s score demonstrated that OFSCs significantly increased the value of left testis reduced by T/D. H&E, hematoxylin and eosin. Analysis of variance with the Tukey–Kramer t test, *P <0.05, n = 6.