Figure 6

Exogenous Jagged1 activation of Notch1 signalling in total and c-Kit^POS/NKX2.5^POS bone marrow mesenchymal stem cells. Total bone marrow mesenchymal stem cells (BMSCs) and c-Kit^POS/NKX2.5^POS BMSCs were classified into Jagged1, IgG, N-(2S-(3,5-difluorophenyl)acetyl)-l-alanyl-2-phenyl-1,1-dimethylethyl ester-glycine (DAPT), dimethyl sulfoxide (DMSO), and MOCK treatment groups (see Methods). After 8 days, activation of Notch1 signalling was assessed by immunofluorescence staining of notch receptor intracellular domain (NICD) and Hes1 protein expression (A) and quantitative RT-PCR analysis of Hes1 mRNA expression (B). For immunofluorescence staining, target proteins were detected with fluorescein isothiocyanate-conjugated IgG. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bar: 50 μm. Representative images were captured by confocal microscopy. Triplicate experiments were performed for real-time quantitative RT-PCR analysis (n = 3). ***P <0.001 versus other groups.