Fig. 2
Systemic human MSC transplantation recovers bone metabolism and IL-17 production in bone marrow of MRL/lpr mice. a, b Osteogenic capacity of recipient BMMSCs. Alizarin Red staining at 4 weeks after osteogenic induction. c, d Osteoclast inductivity of recipient BMMSCs in co-culture with wild-type BL/6 mice-derived bone marrow cells (BMC-WT) under stimulation with 1α, 25-(OH)2 vitamin D3 and prostaglandin E2. TRAP staining. TRAP+ MNCs tartrate-resistant acid phosphate-positive multinucleated cells. e, f Production of interleukin-17 (IL-17) in recipient bone marrow cells (BMCs). e A schema of recipient BMC culture. BMCs were cultured under stimulation with anti-CD3 (Anti-CD3 Ab) and anti-CD28 (Anti-CD28 Ab) antibodies, and then IL-17 was measured in the conditioned medium (CM IL-17). f ELISA of CM IL-17. BMC-hBMMSC bone marrow cells isolated from human bone marrow mesenchymal stem cell-transplanted MRL/lpr mice at 20 weeks of age; BMC-MRL/lpr bone marrow cells isolated from non-transplanted MRL/lpr mice at 20 weeks of age; BMC-Pre-MRL/lpr, bone marrow cells isolated from pre-transplant MRL/lpr mice at 16 weeks of age; BMC-SHED bone marrow cells isolated from stem cells from exfoliated deciduous teeth-transplanted MRL/lpr mice at 20 weeks of age; MSC-hBMMSC bone marrow mesenchymal stem cells isolated from human bone marrow mesenchymal stem cell-transplanted MRL/lpr mice at 20 weeks of age; MSC-MRL/lpr bone marrow mesenchymal stem cells isolated from non-transplanted MRL/lpr mice at 20 weeks of age; MSC-Pre-MRL/lpr bone marrow mesenchymal stem cells isolated from pre-transplant MRL/lpr mice at 16 weeks of age; MSC-SHED bone marrow mesenchymal stem cells isolated from stem cells from exfoliated deciduous teeth-transplanted MRL/lpr mice at 20 weeks of age; Pre-Tx (16w) pre-transplant stage of MRL/lpr mice at 16 weeks of age; Post-Tx (20w) post-transplant stage of MRL/lpr mice at 20 weeks of age. n = 5 for all groups. *P < 0.05, **P < 0.01, ***P < 0.005. Values are shown as means ± SD