Fig. 5

MVs and EPO-MVs inhibited apoptosis of TGF-β treated HK2 cells. Representative photographs of annexinV/PI double-staining in different groups and flow cytometry to test the HK2 cells apoptosis rate after culture in TGF-β1 (6 ng/ml) with MV or EPO-MV at two time points (48 and 72 h) (n = 3 each group). MV and EPO-MV could reduce HK2 cell apoptosis induced by TGF-β1, EPO-MV did better. *p < 0.01, 48-h TGF-β1 group versus HK-2 group or 72-h TGF-β1 group versus HK-2 group, ^$ p < 0.01, 48-h HK2 + TGF-β1 + MV group versus TGF-β1 group or 72-h HK2 + TGF-β1 + MV group versus TGF-β1 group, ^# p < 0.05, 48-h HK2 + TGF-β1 + EPO-MV group versus HK2 + TGF-β1 + MV group or 72-h HK2 + TGF-β1 + EPO-MV group versus HK2 + TGF-β1 + MV. EPO erythropoietin HK2 human kidney 2 MVs microvesicles PI propidium iodide TGF-β1 transforming growth factor-β1