Fig. 2
Neoinnervation of pericardial-derived scaffolds after myocardial infarction. Histological sections of the pericardial-derived scaffold showing nerve fibers (arrows) after Gallego’s modified trichrome (a), light green Masson’s trichrome (b), and hematoxylin/eosin (c) staining. d-d′′ Immunohistological analysis exhibiting positive S100 nerve structures in the scaffold and corresponding zoomed images. e and f Immunofluorescence against βIII tubulin (green), cardiac troponin I (white), and elastin (red) labeled nerve fibers (arrows), myocardium (M) and pericardial-derived bioimplant (P), respectively. Nuclei are counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue) (g and h). Transmission electron microscopy images of the scaffold showing nerve structures composed of Schwann cells (S), axons (A), and fibroblasts of the perineurium (F), endoneurium (E), transport vesicles (TV), microtubules (M), mitochondria (MT), and synaptic vesicles (SV). Scale bars = 100 μm (d), 50 μm (a, b, d′, e, and f), 20 μm (c and d′′), 5 μm (g), and 2 μm (h)