Fig. 1
From: Wnt and Notch signaling pathway involved in wound healing by targeting c-Myc and Hes1 separately
The activities of Wnt and Notch pathways and interaction were analyzed by immunoblot. Wnt activity in wound tissue is increased in response to lithium chloride (LiCl) treatment and is decreased in response to Dickkopf-1 (DKK1) (a, b). Representative immunoblot and results of densitometric analysis of blots (a, b) showed relative levels of Wnt signaling components at the indicated post-wounding time points. Notch activity in wound tissue is increased in response to recombinant human nuclear factor-kappa-B (rhNF-κB) treatment and is decreased in response to N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT) (c, d). Representative immunoblot and results of densitometric analysis of blots (c, d) showed relative levels of Notch signaling components at the indicated post-wounding time points. Wnt and Notch pathways interact during wound healing in wound tissue samples (e-h). Representative immunoblot and results of densitometric analysis of blots (e, f) showed relative levels of Notch signaling components from LiCl-/DKK1-treated and control rats at the indicated post-wounding time points. Representative immunoblot and results of densitometric analysis of blots (g, h) showed relative levels of Wnt signaling components from rhNF-κB-/DAPT-treated and control rats at the indicated post-wounding time points. *P < 0.01, **P < 0.05 compared with the control value (n = 5)