Table 2 Primer sequences and PCR conditions
From: Characterization of bursa subacromialis-derived mesenchymal stem cells
Gene | Oligonucleotide primer sequence | Number of cycles | Annealing temp. (° C) | Product size (bp) |
|---|---|---|---|---|
Verfication of array data | ||||
PRG4 | S: 5′—GCTTGCACCCACCACCACCA—3′ | 38 | 60 | 210 |
A: 5′—AGCTCCTTGGGGGCAGGCTT—3′ | ||||
FGF18 | S: 5′—GTGGGGAAGCCCGATGGCAC—3′ | 35 | 62 | 208 |
A: 5′—GAAGCTCCGGCTGCCCCTTG—3′ | ||||
FGF9 | S: 5′—AATGTGCCCGTGTTGCCGGT—3′ | 35 | 60 | 421 |
A: 5′—GCCTTCCAGTGTCCACGTGCT—3′ | ||||
Meox | S: 5′—CCAACTGGCACCTCCCGCAG—3′ | 37 | 62 | 204 |
A: 5′—CCGCAGGTGACAGTGCCTGG—3′ | ||||
WISP3 | S: 5′—CTGTGTTACATTCAGCCTTGCGAC—3′ | 29 | 54 | 337 |
A: 5′—CTTGGTTTTACAGAATCTTGAGCTC—3′ | ||||
CD200 | S: 5′—TGGCAGCAGTGGTGCTGTGC—3′ | 40 | 60 | 354 |
A: 5′— AGACGGTGAGGCAGGCCGTT—3′ | ||||
BSP | S: 5′—AATGAAAACGAAGAAAGCGAAG—3′ | 33 | 54 | 450 |
A: 5′—ATCATAGCCATCGTAGCCTTGT—3′ | ||||
FOXP2 | S: 5′—AATCGCTGCCTCAAGCTGGC—3′ | 30 | 61 | 493 |
A: 5′—GGTTTGGGCTCTGAGGGTCGC—3′ | ||||
MUC1 | S: 5′— AATGAATGGCTCAAAACTTGG —3′ | 30 | 60 | 231 |
A: 5′— CACTAGGTTCTCACTCGCTCAG —3′ | ||||
Differentiation assays | ||||
Chondrogenic marker genes | ||||
AGN | S: 5′—TGAGGAGGGCTGGAACAAGTACC—3′ | 30 | 54 | 392 |
A: 5′—GGAGGTGGTAATTGCAGGGAACA—3′ | ||||
DEC | S: 5′—AATTGAAAATGGGGCTTTCC—3′ | 27 | 53 | 220 |
A: 5′—GCCATTGTCAACAGCAGAGA—3′ | ||||
FM | S: 5′—CTTACCCCTATGGGGTGGAT—3′ | 35 | 54 | 389 |
A: 5′—GTACATGGCCGTGAGGAAGT—3′ | ||||
SOX9 | S: 5′—ATCTGAAGAAGGAGAGCGAG—3′ | 35 | 58 | 263 |
A: 5′—TCAGAAGTCTCCAGAGCTTG—3′ | ||||
IHH | S: 5′—GAGGAGTCCCTGCATTATGA—3′ | 30 | 54 | 321 |
A: 5′—CAGGAAAATGAGCACATCGC—3′ | ||||
COL II | S: 5′—TTTCCCAGGTCAAGATGGTC—3′ | 35 | 58 | 374 |
A: 5′—CTTCAGCACCTGTCCACCA—3′ | ||||
Osteogenic marker genes | ||||
ALP | S: 5′—TGGAGCTTCAGAAGCTCAACACCA—3′ | 25 | 51 | 454 |
A: 5′—ATCTCGTTGTCTGAGTACCAGTCC—3′ | ||||
COL I | S: 5′—GGACACAATGGATTGCAAGG—3′ | 30 | 54 | 461 |
A: 5′—TAACCACTGCTCCACTCTGG—3′ | ||||
Cbfa1 | S: 5′—ACAGATGATGACACTGCCACC—3′ | 30 | 55 | 324 |
A: 5′—CATAGTAGAGATATGGAGTGCTGC—3′ | ||||
Adipogenic marker genes | ||||
LPL | S: 5′—GAGATTTCTCTGTATGGCACC—3′ | 30 | 51 | 276 |
A: 5′—CTGCAAATGAGACACTTTCTC—3′ | ||||
PPARγ2 | S: 5′—GCTGTTATGGGTGAAACTCTG—3′ | 30 | 51 | 351 |
A: 5′—ATAAGGTGGAGATGCAGGCTC—3′ | ||||
Internal control | ||||
EF1α | S: 5′—AGGTGATTATCCTGAACCATCC-—3′ | 24 | 54 | 234 |
A: 5′—AAAGGTGGATAGTCTGAGAAGC—3′ | ||||