Fig. 4

Concentrated number of endothelial and hematopoietic progenitor cell phenotypes in BMMC. a–d The frequency of cell subpopulations was obtained after labeling of total bone marrow-derived mononuclear cells (BMMC) (1 × 10⁶ cells) separately with antibodies (set 1 CD133/CD34/KDR or set 2 CD34/KDR/CD45) and analysis by flow cytometry. Phenotypes CD34⁺/KDR⁺ (a), CD34⁺/KDR⁺/CD133⁺ (b), CD34/CD45^dim (c) and CD34⁺/KDR⁺/CD45^dim (d) were concentrated in the implanted BMMC of SCD patients. e Concentration factor calculated by the quotient of the specified phenotype number in BMMC/BMA. The high standard deviation indicates the high variability of cell phenotypes within the SCD bone marrow. Data are represented as individuals values. Horizontal bars indicate mean value. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001. BMA bone marrow aspirate