Fig. 3From: A new application of cell-free bone regeneration: immobilizing stem cells from human exfoliated deciduous teeth-conditioned medium onto titanium implants using atmospheric pressure plasma treatmentProtein extraction of Ti surface and relationship between protein and Ca components. Coomassie Brilliant Blue (CBB) (a) and Stains All (b) were used to stain the SDS-PAGE gel after loading the extracts of protein immobilized on titanium powder. Distilled water (DW), 4 M guanidine, or 10 % EDTA was used for extraction. SEM images and SEM-EDX spectrum of 10 % EDTA (c) and 4 M guanidine (d). Bar indicates 1 μm. MW Molecular weight, M Marker, O oxide, P phosphate, Ti titaniumBack to article page