Fig. 7
Bidirectional influence on cell growth between hMSCs and cancer cell lines in co-culture. a hMSCs were co-cultured with the indicated tumor cell lines, and on days 5 and 9 the number of hMSCs and cancer cells from either the co-cultures or from cells cultured alone were enumerated. Data presented as mean ± standard error (SE), n = 3. A fixed number of hMSCs (0.5 × 104) was co-cultured with an increasing number of MCF7 cells (b 1.0 × 103, 0.5 × 104, 1.0 × 104, 0.5 × 105) or HT29 cells (c 1.0 × 103, 0.5 × 104, 1.0 × 104, 0.5 × 105) and the total number of hMSCs or tumor cells was enumerated on day 6. Data presented as mean ± SE, n = 6. d hMSCs (0.5 × 104) were co-cultured with MCF7 (0.5 × 103) or HT-29 (0.5 × 103) cells and the relative hMSC number was enumerated using a SpectraMax/M5 fluorescence spectrophotometer plate reader. Data presented as mean ± SE. Relative number of e HT-29-Luc or f MCF7-Luc number from co-cultures was enumerated using luciferase assay on days 4 and 8. Data presented as mean ± SE, n = 6. MSC mesenchymal (stromal) stem cell; *P <0.05; **P <0.005; ***P <0.0005