Fig. 2

In vitro differentiation of hNPCs into immature and mature neurons. hWJ-MSC CM collected from hypoxic (Hypo) and normoxic (Norm) conditions was able to significantly support and induce neuronal differentiation of hNPCs into b,c immature (DCX⁺ cells) and e,f mature (Map-2⁺ cells) neurons when compared to control conditions (a,d). From g,h cell count analysis it was clearly evident the significant increase of DCX⁺ and Map-2⁺ cell densities in both hWJ-MSC CM (from normoxic and hypoxic conditions) when compared to the control group (values are shown as mean ± SEM. **p < 0.01). Scale bar: 100 μm. CTR control, DAPI 4-6-diamidino-2-phenylindole-dihydrochloride, DCX doublecortin, MAP-2 microtubule associated protein-2