Fig. 3

STAT3 is activated in hypo-ECFCs under ischemia. a Western blot analysis was performed to determine the levels of p-STAT3 in hindlimb ischemia injury after transplantation of hypo-ECFCs. b Co-immunofluorescence staining was used to detect p-STAT3 and hypo-ECFCs (human nuclear antigen (HNA)-positive cells, red). DAPI (blue) was used for nuclear staining. White color indicates merged color with green and red colors. Arrows indicate p-STAT3⁺/HNA⁺/DAPI⁺ cells. ECFC endothelial colony-forming cell, PBS phosphate-buffered saline