Fig. 2
SIMC quantification of H3K27me3-K36me2 and H3K27me2-K36me3-containing peptides by MS/MS. S1 (K27me2-K36me3) and S2 (K27me3-K36me2) of H3K27-R40me5, and their mixtures M1–M4, were subjected to LC-MS/MS analysis. a–f The intensity of two types of y5–132+ in multiple MS/MS spectra of all samples was summed up and displayed as a SIMC profile. g SIMC quantification of K27me3-K36me2 (green) and K27me2-K36me3 (red) in H3K27-R40me5. The MS experiment was repeated three times and the error bars represent the SD. SIMC specific ions of isobaric modification chromatogram