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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Cis-existence of H3K27me3 and H3K36me2 in mouse embryonic stem cells revealed by specific ions of isobaric modification chromatogram

Fig. 3

SIMC quantification of tryptic K27me2-K36me3 and K27me3-K36me2-containing H3L20-R52me5 peptides. a Schematic representation of the isobaric H3L20-R52me5 peptides. b SIMC quantification of K27me3-K36me2 (green) and K27me2-K36me3 (red) in H3L20-R52me5. L1 (K27me2-K36me3) and L2 (K27me3-K36me2) of H3L20-R52me5 with different ratios (3:1, 1:1, 1:3, and 1:6) were digested by trypsin for 20, 40, and 120 minutes, respectively, and were analyzed by LC-MS/MS. The MS experiment was repeated three times and error bars represent the SD

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Stem Cell Research & Therapy

ISSN: 1757-6512