Fig. 1
H2O2-induced apoptosis. a Cell survival was evaluated after treating cells with increasing concentrations of H2O2 for 6 hours (***p <0.0001, **p <0.001). b Expression of cleaved caspase-3 and PARP was measured by western blotting in cells treated with increasing concentrations of H2O2 for 6 hours (*p <0.05). c Schematic presentation of miRNA binding site in the 3′ UTR of human caspase-3. Candidate miRNAs predicted to target caspase-3 were selected based on the TargetScan miRNA-target prediction database [19]. miRNAs with an aggregation Pct value ≥0.2 were selected. d Cell survival was measured in candidate miRNA-transfected hMSCs. All samples were treated with 500 μM H2O2 after miRNA transfection. e Effect of H2O2 on endogenous let-7b expression was evaluated by real-time PCR. Quantitative data expressed as mean ± standard deviation of at least three independent experiments. (*p <0.05, and #p <0.05)