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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Stem cells isolated from human dental pulp and amniotic fluid improve skeletal muscle histopathology in mdx/SCID mice

Fig. 2

Immunofluorescent analysis after 2 and 4 weeks of myogenic differentiation of hDPSCs and hAFSCs by demethylation treatment. Immunofluorescent staining with anti-myogenin (green)/anti-MyHC (red) and anti-myogenin (green)/anti-desmin (red) antibodies. Myogenic differentiation was induced with and without the addition of CM from differentiated C2C12 cultures. Human DPSCs and AFSCs underwent myogenic commitment as early as after 14 days of induction (a) by expressing the muscle-specific markers myogenin, MyHC, and desmin. However, multinucleated myotube formation occurred only after 4 weeks of induction (b). Scale bars = 50 μm. CM conditioned medium, hAFSC human amniotic fluid stem cell, hDPSC human dental pulp stem cell, MyHC myosin heavy chain

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Stem Cell Research & Therapy

ISSN: 1757-6512