Fig. 5

Analysis of typical pluripotency (PP) markers a–f and methylation of Oct-4 promoter g–j. Expression of PP markers could be confirmed (2^–ΔΔCt vs. skin), primarily in early passages a, b. In general, Oct-4A represented the highest levels a, whereas Klf4 levels were very low f. As expected, all pluripotency marker levels decrease over time. Interestingly, marmoset tissues display significantly lower Sox2 levels vs. human f, but also significantly higher Lin28A levels e. *Significant change (Student’s t test). The nature of all PCR products was confirmed by DNA sequencing. Methylation analysis of the Oct-4 promoter region revealed the heterogeneity within the cell populations: marmoset amnion MSCs appeared to contain the most cells with an unmethylated pattern (open circles) g, whereas human amnion cells contained more methylated (black circles) populations i