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Table 1 Ex vivo pattern of expression of individual markers on CD13high CD105+ CD45 cells from normal/reactive bone marrow samples

From: Ex vivo identification and characterization of a population of CD13high CD105+ CD45 mesenchymal stem cells in human bone marrow

Code

Antigen

Expression pattern

% of positive cellsa

Amount of protein expressed/cellb

(% of positive cases; positive cases/total cases)

CD10

Neprilysin

−/+

89 %

6916

(100 %; 18/18)

(62–97 %)

(1883–22,177)

CD11b

Integrin alpha-M

NA

NA

(0 %; 0/9)

CD13

Aminopeptidase N

++/+++

100 %

122,414

(100 %; 60/60)

(100–100 %)

(45,582–260,438)

CD14

Myeloid Cell-Specific Leucine-Rich Glycoprotein

NA

NA

(0 %; 0/47)

CD19

B-Lymphocyte Surface Antigen B4

NA

NA

(0 %; 0/37)

CD34

Hematopoietic Progenitor Cell Antigen-1

NA

NA

(0 %; 0/60)

CD45

Leukocyte common antigen

NA

NA

(0 %; 0/60)

CD73

Ecto-5′-nucleotidase

+/++

100 %

7816

(100 %; 58/58)

(100–100 %)

(3268–23,090)

CD90

Thy-1

+/+++

100 %

21,875

(100 %; 55/55)

(100–100 %)

(1870–260,738)

CD105

Endoglin

+

100 %

2463

(100 %; 60/60)

(100–100 %)

(700–4620)

CD117

Kit

NA

NA

(0 %; 0/54)

CD146

Melanoma cell adhesion molecule

+Low

100 %

547

(100 %; 55/55)

(100–100 %)

(267–3054)

NA

HLA-DR

−/+

91 %

3296

(100 %; 34/34)

(50–100 %)

(390–15,823)

  1. Codes used for the intensity of expression of individual markers: (−) negative; (−/+) heterogeneous from negative to positive; (+Low) dim positive (median fluorescence intensity (MFI) ≥250 and <1000); (+) moderate positive (MFI ≥1000 and <10,000); (++) positive (MFI ≥10,000 and <50,000); (+++) strong positive (MFI ≥50,000). Mixed symbols indicate variable reactivity, either among different samples or among cells from the same sample
  2. aResults expressed as median (range)
  3. bNormalized MFI values (median (range)) calculated after subtracting the background autofluorescence levels observed for each individual marker-associated fluorochrome and expressed as arbitrary units scaled from 0 to 262,000
  4. NA not applicable