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Table 1 Ex vivo pattern of expression of individual markers on CD13high CD105+ CD45 cells from normal/reactive bone marrow samples

From: Ex vivo identification and characterization of a population of CD13high CD105+ CD45 mesenchymal stem cells in human bone marrow

Code Antigen Expression pattern % of positive cellsa Amount of protein expressed/cellb
(% of positive cases; positive cases/total cases)
CD10 Neprilysin −/+ 89 % 6916
(100 %; 18/18) (62–97 %) (1883–22,177)
CD11b Integrin alpha-M NA NA
(0 %; 0/9)
CD13 Aminopeptidase N ++/+++ 100 % 122,414
(100 %; 60/60) (100–100 %) (45,582–260,438)
CD14 Myeloid Cell-Specific Leucine-Rich Glycoprotein NA NA
(0 %; 0/47)
CD19 B-Lymphocyte Surface Antigen B4 NA NA
(0 %; 0/37)
CD34 Hematopoietic Progenitor Cell Antigen-1 NA NA
(0 %; 0/60)
CD45 Leukocyte common antigen NA NA
(0 %; 0/60)
CD73 Ecto-5′-nucleotidase +/++ 100 % 7816
(100 %; 58/58) (100–100 %) (3268–23,090)
CD90 Thy-1 +/+++ 100 % 21,875
(100 %; 55/55) (100–100 %) (1870–260,738)
CD105 Endoglin + 100 % 2463
(100 %; 60/60) (100–100 %) (700–4620)
CD117 Kit NA NA
(0 %; 0/54)
CD146 Melanoma cell adhesion molecule +Low 100 % 547
(100 %; 55/55) (100–100 %) (267–3054)
NA HLA-DR −/+ 91 % 3296
(100 %; 34/34) (50–100 %) (390–15,823)
  1. Codes used for the intensity of expression of individual markers: (−) negative; (−/+) heterogeneous from negative to positive; (+Low) dim positive (median fluorescence intensity (MFI) ≥250 and <1000); (+) moderate positive (MFI ≥1000 and <10,000); (++) positive (MFI ≥10,000 and <50,000); (+++) strong positive (MFI ≥50,000). Mixed symbols indicate variable reactivity, either among different samples or among cells from the same sample
  2. aResults expressed as median (range)
  3. bNormalized MFI values (median (range)) calculated after subtracting the background autofluorescence levels observed for each individual marker-associated fluorochrome and expressed as arbitrary units scaled from 0 to 262,000
  4. NA not applicable