Fig. 8
Flow cytometry analysis of the rapid in vitro interaction of HSCs with Ad-MSCs. a Representative flow cytometry duplet analysis of 6×105 CFSE-stained Ad-MSCs and 1500 DiD-stained LSK cells, 10 minutes after co-culture. b Percentage of DiD+LSK cells forming duplets with CFSE+ Ad-MSCs detected in the different assays. c Multi-spectral images of PE+ LSK cells and FITC+ Ad-MSCs. Individual cells and three representative duplets are shown by a row of images that include side scatter signal (blue), FITC signal (green), PE signal (orange), and FITC and PE signals together (merged column). Bright-field (BF) of each cell sample is also represented. Ad-MSC adipose tissue-derived mesenchymal stem cell, CFSE carboxyfluorescein succinimidyl ester, DAPI 4',6-diamidino-2-phenylindole, FITC fluorescein isothiocyanate, FSC forward scatter, HSC hematopoietic stem cell, LSK lineage− Sca-1+ cKit+, PE phycoerythrin, SSC side scatter