Fig. 3

The CB1 antagonist AM251 blocks the rescue of mEPSC changes sustained by LPS⁺aNPCs in TNFα-treated cultures. a Representative electrophysiological traces of mEPSCs under the different treatment conditions. b, c Group data of average mEPSC amplitude and frequency of untreated (n = 15), TNFα-treated (n = 13), LPS⁺aNPCs + TNFα (n = 10), and LPS⁺aNPCs + TNFα + AM 251-treated (n = 9) cells. LPS⁺aNPC treatment fails to revert TNF effect in the presence of AM 251 (P < 0.05, P < 0.01). d Confocal microscopy images showing expression of CB1 receptor in cultured cortical neurons. Immunofluorescence for CB1 (green), VGAT (red), and Merge in the upper panels and for CB1 (green), VGLUT (red) and Merge in lower panels is shown. Scale bars, 20 μm. e Summary bar graphs showing the colocalization of CB1 and VGAT or VGLUT. All data are expressed as mean ± standard error of the mean. Statistical test: one-way analysis of variance, Dunn’s multiple comparisons test. CB1 type 1 cannabinoid receptor, LPS ⁺ aNPC lipopolysaccharide-stimulated adult neural stem cell, mEPSC miniature excitatory post-synaptic current, TNFα tumor necrosis factor alpha