Fig. 5

Effects of miR-17 overexpression on adhesion and migration of CB CD34⁺ cells. a The adhesion of miR-17 overexpressing (17/OE) CB CD34⁺ cells to N-cadherin or vascular cell adhesion molecule-1 (VCAM1) was significantly increased compared with that of control (CRTL) cells. Results are shown as mean + SD (n = 8 for N-cadherin and n = 10 for VCAM1). *p < 0.05, between 17/OE and CTRL groups (Student’s t-test). b The adhesion of 17/OE CD34⁺ cells with β₁-integrin deficient (β₁/KD) to VCAM1 was significantly decreased compared with that of CRTL cells. Mean + SD is shown (*p < 0.05, Student’s t-test). The CTRL is 17/OE CD34⁺ cells with scrambled shRNA. c The specific adhesion of CD34⁺ cells to N-cadherin or VCAM1 was confirmed by antibody blocking experiments. The adhesion was measured as described above. The adhesion of pre-treated cells was significant inhibited compared with that of non-treated cells (*p < 0.05, Student’s t-test). d The migration of 17/OE CB CD34⁺ cells towards SDF1α was slightly increased compared with that of CTRL cells, but statistical analyses indicated it did not meet statistical significance (p > 0.3, Student’s t-test)