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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Osteogenic differentiation of mesenchymal stromal cells in two-dimensional and three-dimensional cultures without animal serum

Fig. 7

Detection of mineralized matrix and calcium deposition in three-dimensional (3D) cell culture. Cells were cultured in 3D matrices and fixed on day 28, mounted into Tissue-Tek and cut into 6 μm sections that were stained with Alizarin Red and photographed (a). Deposited calcium was quantified on days 7, 14 and 21 of culture (b). DIFF differentiation, FCS fetal calf serum, PLP platelet lysate and plasma

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Stem Cell Research & Therapy

ISSN: 1757-6512