Fig. 1

Regulation of BM-MSC migration by inflammatory molecules. Scratch test assay was performed on BM-MSC isolated from three different donors and seeded in plates coated with type I dermal collagen, cellular fibronectin, or vitronectin or without ECM. Pictures of scratches were taken immediately (T = 0) or 22 hours after culture (T = 22 h) in serum-free control medium or medium supplemented with IL-1β, IL-8, TNFα, or PDGF-AB. a Quantification of BM-MSC migration. Results expressed as percentages of wound closure at T = 22 h compared with T = 0 for each condition. Data expressed as mean ± SEM of three independent experiments (one donor per experiment), each performed in triplicate. *P <0.05. b, c One experiment representative of the three experiments is shown. ECM extracellular matrix, IL interleukin, PDGF-AB platelet-derived growth factor AB, TNFα tumor necrosis factor alpha