Fig. 6

adCD-MSCs can be efficiently labeled by Nile Red staining. Fluorescence intensity and distribution of Nile Red staining. a–d Representative micrograph of stained cell cultures and e–h three-dimensional histograms of confocal analysis of fluorescence intensity (expressed as fluorescence intensity/pixel) for each time point. Fluorescence intensity of Nile Red-labeled adCD-MSCs analyzed by flow cytometry is high at i 15 hours, l 24 hours, and m 72 hours and n decreased significantly at 120 hours post labeling. Magnification 40×; scale bar, 20 μm