Fig. 7

In vivo administration of Nile Red-labeled adCD-MSCs in DSS-treated colitic mice. DSS-treated mice were subjected to enema administration of Nile Red-stained adCD-MSCs. Colon tissue sections were collected 24 hours after the last MSC administration and were analyzed by confocal or optical microscopy. a DSS-treated mice colon sections show a low fluorescence background, in the absence of adCD-MSCs. Fluorescence signals in the presence of b Nile Red-stained adCD-MSCs or c adCD-MSCs/hCBPL. d Sections from consecutive segment were fixed and stained with H&E. e Merged images show that Nile Red fluorescence is present mainly in the muscular layers surrounding the colon injury. f Fluorescence quantification is presented as mean fluorescence intensity (± SEM). Data are representative of three mice for each experimental group and 15 acquired sections for each experimental condition. *p <0.05. Magnification 20×; scale bar, 150 μm. adCD-MSC mesenchymal stromal cell isolated from adipose tissue of Crohn’s disease patients, DSS dextran sulfate sodium, hCBPL human umbilical cord blood-derived platelet lysate, PBS phosphate saline buffer