Fig. 3

rMAPC suppress antigen-specific T-cell proliferation and IFNγ production. a Co-culture of MBP-specific T cells with rMAPC in increasing ratios (1:0.5 to 1:2). Results are shown as percentage of proliferation normalized to the positive control (1:0, T cells + MBP). Mean values ± SEM are from three independent experiments, with triplicates per experiment. Negative control (T cells without MBP) is shown as a dotted line. b IFNγ levels measured in the co-culture supernatants with enzyme-linked immunosorbent assay. Results are presented as percentages normalized to the positive control (1:0, T cells + MBP). Mean values ± SEM are from three experiments, with triplicates per experiment. c Analyses of NO levels in co-culture supernatants through the measurement of nitrite. Results are shown as percentages normalized to the lowest ratio in the co-culture (1:0.5). Mean values ± SEM are from three independent experiments, with triplicates per experiment. Nitrite was not detected (ND) in the positive control (1:0, T cells + MBP), or in thymocytes alone + MBP (not shown). Non-parametrical Kruskal Wallis multiple group comparison test was used followed by Dunns test for differences between groups. Significant differences with the control condition are indicated with asterisks: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. IFN interferon, MBP myelin basic protein, NO nitric oxide