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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Differentially expressed microRNAs in bone marrow mesenchymal stem cell-derived microvesicles in young and older rats and their effect on tumor growth factor-β1-mediated epithelial-mesenchymal transition in HK2 cells

Fig. 1

Characterization of young and old MSCs as well as young and old MVs. a BM-MSCs from young and old Fisher344 rats were cultured to passage 3 and labeled with a FITC-coupled antibodies against CD44 and CD146 and a PE/Cy7-coupled antibody against CD45 and CD105 or immunoglobulin isotype control IgGs and analyzed by using FACS. b MVs from young and old MSCs were collected and labeled with FITC-coupled antibodies against CD44, a4-integrin, and PE/Cy7-coupled antibody against CD29 or immunoglobulin isotype control IgGs and analyzed by using FACS. c Representative photomicrographs show lipid inclusions with Oil Red O (left) and mineralization with alizarin red (right) in MSCs from young (top) and old rats (bottom). FACS fluorescence-activated cell sorting, FITC fluorescein isothiocyanate, MSC mesenchymal stem cell, MV microvesicle, PE phycoerythrin

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Stem Cell Research & Therapy

ISSN: 1757-6512