Fig. 2

ECIS measurement of increased Transwell filter resistance resulting from SDF-1 chemokine-dependent Jurkat T-cell invasion of Matrigel-coated Transwell filters. Jurkat T cells were placed in the upper chambers of Matrigel-coated Transwells with or without addition of 100 ng/ml SDF-1 to the lower chambers. a Transwell filter resistance is measured continuously over time by ECIS. Combined resistance tracings of six separate experiments are shown for Transwells with and without the addition of SDF-1 to the lower chamber, demonstrating the increased resistance in Transwells where the Jurkat T cells invade the Matrigel in response to SDF-1. Arrow: time point of SDF-1 addition to test lower chambers. Mean ± SEM. b The absolute change in ECIS Transwell filter resistance, relative to the resistance measured at the zero time point, increases over 2 hours due to Jurkat T-cell Matrigel invasion in response to SDF-1. Each bar represents the mean ± SEM of six separate experiments (n = 6) performed in duplicate. *p <0.05. SDF-1 stromal cell derived factor-1