Fig. 3
From: Rapid assay of stem cell functionality and potency using electric cell-substrate impedance sensing
Increased ECIS Transwell filter resistance is due to chemotaxis, not chemokinesis, and is specific to the chemokine (SDF-1). In these experiments, Jurkat T-cells were used without pretreatment or were pretreated with either 100 ng/ml SDF-1 or AMD3100 (an inhibitor of the SDF-1 receptor). Resistance was measured in wells with Jurkat T cells (without or with pretreatment) added to top chambers with and without SDF-1 in the bottom chambers. Arrow: time point of SDF-1 addition to test lower chambers. Each tracing represents the mean ± SEM of two separate experiments (n = 2) performed in duplicate. SDF-1 stromal cell derived factor-1