Fig. 2

SIRT1 is a direct target of posttranscriptional repression by miR-34a. a The predicted targeting sites with miR-34a of SIRT1 3′-UTR (Hsa, human; Mmu, mouse) are highlighted in red. b qRT-PCR analysis was applied to detect mRNA expression of SIRT1 in MSCs after transfection with miR-34a mimic, NC mimic, miR-34a inhibitor, or NC inhibitor for 48 hours, respectively. c, d Western blot analysis showed dose-dependent regulation of SIRT1 by miR-34a after transfection with miR-34a mimic, NC mimic, miR-34a inhibitor, or NC inhibitor for 72 hours, respectively. Each column represents mean ± SD from three independent experiments. *P <0.05 vs. control, △P <0.05 vs. transfection with 10 nM miR-34a mimic. miRNA microRNA, NC negative control, ORF open reading frame, SIRT1 silent information regulator 1, UTR untranslated region