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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Differential ability of MSCs isolated from placenta and cord as feeders for supporting ex vivo expansion of umbilical cord blood derived CD34+ cells

Fig. 3

CD34+ cells expanded on P-MSCs have augmented in vitro functionality of the expanded cells. a P-MSCs:CD34+ co-cultures displayed higher blast-forming unit erythroid (BFU-E), granulocyte–monocyte (GM), granulocyte-erythroid-monocyte megakaryocyte (GEMM), and megakaryocytes (MK) colonies when compared with C-MSCs as feeders. b P-MSCs as feeders maintained significantly higher LTC-IC units than C-MSCs. c Trans well migration assay displayed significantly augmented migration of the expanded cells towards SDF-1α in the P-MSCs set as compared to the C-MSCs set. d Migrated fraction from the co-culture with P-MSCs revealed significantly higher numbers of primitive CD34+CD38−cells. e Superior migratory response might be attributed to the higher percentage of CD34+CXCR-4+ cells in co-cultures with P-MSCs as feeders. f Quantitation of the attachment to the fibronectin was carried out after lysing the adhered cells and measuring color intensity at 570 nm. The inset shows sort-purified CD34+ expanded on P-MSCs adhered to fibronectin in higher numbers. Data are represented as mean ± standard deviation from three independent sets of experiments. *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001. P-MSCs placenta-derived mesenchymal stem cells, C-MSCs cord-derived mesenchymal stem cells, LTC-IC long term culture initiating cell

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