Fig. 6

Retention of mADSC after subcutaneous transplantation of CS or intramuscular injection of dispersed cells. Prior to transplantation CS were labeled by CMFDA (green) and dispersed mADSC – by CM-Dil (red) vital dyes. At day 14 after transplantation animals were sacrificed and skeletal muscle samples were used for detection of ADSC retention at the site of delivery (on the surface of the femoral quadriceps muscle for CS or within the anterior tibia muscle for injected mADSC). In the CMFDA-labeled CS, DAPI stain was omitted due to significant signal quenching after washing of fresh sections in saline or fixation. Corresponding sections were stained with H & E to evaluate tissue status. mADSC mouse adipose-derived stromal cells, CS cell sheet, CMFDA 5-chloromethylfluorescein diacetate, DAPI 4′,6-diamidino-2-phenylindole, H & E, hematoxylin and eosin