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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Transplantation of rat embryonic stem cell-derived retinal progenitor cells preserves the retinal structure and function in rat retinal degeneration

Fig. 5

Subretinal transplantation of EGFP labeled rESC-RPCs in the RCS model. a Generation of rESC-RPC2 from EF1α::EGFP lentivirus labeled rESCs. Scale bars: 50 μm. b Immunofluorescence staining of EGFP-rESC-RPC2 at D16 before transplantation. Antibody against EGFP (green) and antibodies against Nestin/Nes and Tuj1 (red) were used. DAPI (blue) was used to highlight the nuclei. Scale bars: 50 μm. c Flow cytometric analysis of EGFP-rESC-RPC2 in comparison with unlabeled rESC-RPC2. d Grafted rESC-RPC2 could be detected in the retina at different times after transplantation. GCL ganglion cell layer, INL inner nuclear layer, ONL outer nuclear layer. Scale bar: 50 μm. EGFP enhanced green fluorescent protein, rESC rat embryonic stem cell, RPC retinal progenitor cell, RCS Royal College of Surgeons, DAPI 4′,6-diamidino-2-phenylindole

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