Fig. 3
From: Porcine lung mesenchymal stromal cells possess differentiation and immunoregulatory properties
Differentiation potential of porcine L-MSCs. a Adipocyte differentiation. L-MSCs when cultured in adipogenic medium for 21 days showed lipid droplets in the cytoplasm of differentiated cells. b No adipocyte differentiation was detected in cells cultured in DMEM. c Osteocyte differentiation. L-MSCs cultured in osteogenic medium for 21 days showed calcium deposition as detected by Von Kossa staining. d No osteogenic differentiation was observed in cells cultured in DMEM. e–h Epithelial differentiation. L-MSCs cultured in epithelial differentiation medium for 10 days exhibited cuboidal morphology (e) and were found to express epithelial markers pan-cytokeratin (g) and cytokeratin-18 (i) whereas L-MSCs cultured in DMEM displayed normal spindle-shaped morphology (f), and expression of pan-cytokeratin (h) and cytokeratin-18 (j) was not detected on undifferentiated L-MSCs