Fig. 5
From: Transplantation of hESC-derived hepatocytes protects mice from liver injury
VAL9- HEP can rescue acetaminophen (APAP)-induced acute liver failure (ALF). a Representative images of transduced VAL9-HEP (phase-contrast and fluorescence) for green fluorescent protein (GFP). b Flow cytometry analysis of GFP expression from transduced VAL9-HEP 2 days after transduction with A1AT-GFP lentivector. The numbers indicate the percentages of GFP-positive cells in the analyzed gate. c Representative histopathology of livers of control, acetaminophen-treated mice and acetaminophen-treated mice after transplantation with VAL9-HEP. d Survival curves of immunodeficient mice (n = 10 in each group) that received intrasplenic cell transplantation with 1.0 × 106 VAL9-derived hepatocytes. e Secreted ALB in the serum of transplanted animals at different time points. f Liver function test after infusion of acetaminophen then saline or VAL9-HEP at different time points. *p < 0.05, **p < 0.01 versus control; # p < 0.05 versus APAP-treated mice. ALT Alanine aminotransferase, AST Aspartate aminotransferase