Human adult stem cells derived from adipose tissue and bone marrow attenuate enteric neuropathy in the guinea-pig model of acute colitis

Table 2 Effects of mesenchymal stem cells derived from bone marrow and adipose tissue on myenteric neurons in TNBS-induced colitis

	Sham	TNBS	TNBS + BM-MSC	TNBS + AT-MSC
	Total number of myenteric neurons/2 mm²
24 h	263.8 ± 3.5	202.5 ± 8.4 ††††	258.8 ± 2.9 ****	242.8 ± 6.2 *** †
72 h	262.0 ± 5.1	204.0 ± 5.4 †††	258.8 ± 7.6 **	240.5 ± 10.0 ** †
	Total number of nNOS-IR neurons/2 mm²
24 h	52.3 ± 1.8	70.0 ± 4.9 †††	55.0 ± 3.2 **	53.5 ± 2.6 **
72 h	52.5 ± 1.0	70.5 ± 3.2 †††	55.3 ± 2.7 **	57.3 ± 1.7 *
	Proportion of nNOS-IR neurons/2 mm² (%)
24 h	19.9 ± 0.9	34.8 ± 2.8 ††††	21.3 ± 1.2 ****	22.1 ± 1.1 ****
72 h	20.2 ± 0.3	34.6 ± 1.8 ††††	21.4 ± 1.3 ****	23.9 ± 0.7 ***
	Total number of ChAT-IR neurons/2 mm²
24 h	163.5 ± 2.1	111.3 ± 5.7 ††††	148.3 ± 4.6 ****	144.5 ± 4.9 *** †
72 h	157.8 ± 6.3	112.3 ± 1.7 ††††	147.5 ± 7.3 ****	145.3 ± 3.4 ***
	Proportion of ChAT-IR neurons/2 mm² (%)
24 h	62.0 ± 1.4	55.1 ± 2.9	57.3 ± 1.6	59.5 ± 0.9
72 h	59.1 ± 0.9	55.2 ± 2.3	57.1 ± 3.0	60.7 ± 2.5

TNBS 2,4,6-trinitrobenzene sulfonic acid, BM-MSC bone marrow-derived MSC, AT-MSC adipose tissue-derived MSC, MSC mesenchymal stem cell, nNOS neuronal nitric oxide synthase, ChAT choline acetyltransferase, IR immunoreactive
*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, significantly different to TNBS; †p < 0.05, †††p < 0.001, ††††p < 0.0001, significantly different to sham

ISSN: 1757-6512