Fig. 1

Individual or pooled donor polyclonal T-cell proliferation. a Mean ± SD proliferation of five random single donor buffy coat-derived CFSE-labeled peripheral blood mononuclear cells (PBMCs) in the absence (grey bars) or presence of phytohemagglutinin (+PHA, green bars) after 4 or 7 days indicating significant donor variation. b Simultaneous inhibition of mitogenic and alloimmunogenic T-cell proliferation by umbilical cord (UC)-derived MSPCs was tested. CFSE pre-labeled cryopreserved pooled PBMCs (pPBMCs) were seeded in the absence of MSPCs without (grey bars) or with PHA (green bars) or in the presence of UC-MSPC without (light red bars; inhibition of allo-MLR) or with PHA (red bars; inhibition of additional mitogenesis) at an MSPC:PBMC ratio of 1:3. The time course of proliferation between days 4 to 7 is shown in Additional file 2 (Figure S1C). Mean ± SD results from representative experiments (n = 5 in a and b). Significant differences are indicated (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)