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Table 1 Percent positive cells obtained from cultures on the various substrates

From: Native extracellular matrix preserves mesenchymal stem cell “stemness” and differentiation potential under serum-free culture conditions

   SSEA-4 CD73 CD90 CD105 CD146
Time in culture Culture substrate      
4 days TCP 29 ± 4 45 ± 4 45 ± 5 50 ± 4 24 ± 4
  CELLstart™ 46 ± 5 50 ± 7 49 ± 4 47 ± 5 59 ± 7
  BM-ECM 64 ± 6 67 ± 7 67 ± 5 65 ± 6 68 ± 7
7 days TCP N/A N/A N/A N/A N/A
  CELLstart™ 45 ± 7 44 ± 8 61 ± 8 46 ± 9 65 ± 8
  BM-ECM 81 ± 10 80 ± 6 81 ± 7 70 ± 6 83 ± 9
  1. TCP tissue culture plastic, BM-ECM bone marrow-derived extracellular matrix, N/A not applicable
  2. Early passage (P1) cultures of BM-MSCs were grown on TCP, BM-ECM, or CELLstart™ in SFM for 4 and 7 days. Phenotypic expression of MSC-associated markers (SSEA-4, CD73, CD90, CD105, and CD146) was assessed by using flow cytometry. The data are the percent positive cells of the total cell population expressing each marker. The experiment was performed in triplicate, and the mean ± standard deviation is shown. Each experiment was performed three times