Fig. 3

Histologic analysis of spinal cord lesions stained with H&E and Luxol fast blue. Control group a–c, MPSS group d–f, AD-MSCs group g–i, and AD-MSCs + MPSS group j–l were observed. In the H&E staining a, d, g, j, all groups showed severe hemorrhage and infiltration of microglial cells in the injured part of the spinal cord. The injured parenchyma of spinal cord was composed of demyelinated neurons, cell debris, mild fibrosis, and hemorrhage in the high-powered field b, e, h, k. The AD-MSCs group showed less hemorrhaging and fewer inflammatory responses compared with other groups. In addition, all groups exhibited severe demyelination of nerve fibers in the SCI lesion stained with Luxol fast blue c, f, i, l. Bar: a, d, g, j 200 μm; b, c, e, f, h, i, k, l 25 μm